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Monday, October 5, 2020 | History

2 edition of The Response of a human bronchial epithelial cell line to histamine found in the catalog.

The Response of a human bronchial epithelial cell line to histamine

The Response of a human bronchial epithelial cell line to histamine

intracellular calcium changes and extracellular release of inflammatory mediators

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  • 23 Currently reading

Published by U.S. Environmental Protection Agency in [Washington, D.C .
Written in English

    Subjects:
  • Epithelial cells.,
  • Histamine.

  • Edition Notes

    StatementTerry L. Noah ... [et al.].
    ContributionsNoah, Terry L., United States. Environmental Protection Agency.
    The Physical Object
    FormatMicroform
    Pagination[9] p.
    ID Numbers
    Open LibraryOL14693302M

      Human neutrophil elastase induces hypersecretion of mucin from well-differentiated human bronchial epithelial cells in vitro via a protein kinase C{delta}-mediated mechanism. The American journal Author: Ayşe Kılıç, Asher Ameli, Asher Ameli, Jin-Ah Park, Alvin T. Kho, Kelan Tantisira, Marc Santolini, Fe. Phenotyping and cytokine regulation of the BEAS-2B human bronchial epithelial cell: demonstration of inducible expression of the adhesion molecules VCAM-1 and ICAM Am J Respir Cell Mol Biol Cited by: 1.

    Human β-Defensin 1, 2 and 3 Enhance TGF-β1-Mediated Epithelial-Mesenchymal Transition (EMT) in Bronchial Epithelial Cells Xiao-Yan Qu, Xiao-Min Dang, Dong Shang, Wei-Jia Wang, Dan Xu, Yong-Ping Shao, Wu-Yuan Lu, and Ying Chang. Two airway cell types are critical for asthma pathogenesis: epithelial cells and smooth muscle cells. Airway epithelial cells, which are the first line of defense against inhaled pathogens and particles, initiate airway inflammation and produce mucus, an Author: David J. Erle, Dean Sheppard.

      BEAS-2B human airway epithelial cells (AEC) profoundly inhibit both constitutive and IgE-dependent human lung mast cell (HLMC) histamine release. The aim of this study was to examine the regulation of HLMC degranulation by primary AEC from healthy and asthmatic subjects, and investigate further the inhibitory by: Ca2+ transients evoked by endothelin-1 (ET-1) were measured in single cells of an immortalized human tracheal epithelial cell line using Fura ET-induced Ca2+ transients were compared to signals evoked via established phospholipase-C linked Cited by: 8.


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The Response of a human bronchial epithelial cell line to histamine Download PDF EPUB FB2

The response of a human bronchial epithelial cell line to histamine: intracellular calcium changes and extracellular release of inflammatory mediators. Noah TL(1), Paradiso AM, Madden MC, McKinnon KP, Devlin by: The Response of a human bronchial epithelial cell line to histamine: intracellular calcium changes and extracellular release of inflammatory mediators Author: Terry L Noah.

Histamine has a key role in allergic inflammatory conditions. The inflammatory responses resulting from the liberation of histamine have long been thought to be mediated by the histamine H 1 receptor, and H 1 -receptor antagonists — commonly known as antihistamines — have been used to treat allergies for many by: In order to study the effect of histamine on paracellular epithelial permeability and tight junctional integrity, we have cultured human bronchial epithelial cells to confluency and investigated the effect of topically applied microM histamine.

Vignola AM, Campbell AM, Chanez P, et al. Activation by histamine of bronchial epithelial cells from nonasthmatic subjects. Am J Respir Cell Mol Biol ; 9: – Am J Respir Cell Mol Biol ; 9: – Using the human lung epithelial WI26VA4 cell line, we investigated the capacity of fenspiride, an anti-inflammatory drug with anti-bronchoconstrictor properties, to interfere with histamine-induced intracellular Ca 2+ increase and eicosanoid by: 3.

response to bacterial LPS in airway epithelial cells [16HBE14o- and primary nasal epithelial cells (PNECs)]. Methods Cell culture The immortalized bronchial epithelial cell line 16HBE14o-(obtained from D. Gruenert UCSF, USA) was grown as described previously (Kelly et al., c). PNECs were obtained from healthy volunteers (n = 7) and grown as Cited by:   Background: Airway epithelial cells form the first line of defense against B.

cenocepacia infection. Results: Airway epithelial cells express low levels of caspase-1 and do not release processed IL-1β. Conclusion: Low level expression of caspase-1 results in the inability of airway epithelial cells to secrete IL-1β.

Significance: This study provides novel insight into airway epithelial cell. Co-culture of human eosinophils with BEAS-2B cells. The human bronchial epithelial cell line BEAS-2B cells were obtained from the American Type Culture Collections (Manassas, VA, USA) and Cited by: 5. Optimally, a balanced Th1 and Th2 response is suited to the immune challenge, and a dysregulated response is linked to a variety of chronic inflammatory conditions like asthma and chronic bronchitis.

11 CD8+ T cells are mainly cytotoxic T cells. They secrete molecules that kill infected cells and tumor by: Histamine is a biogenic amine with extensive effects on many cell types, including important immunologic cells, such as antigen-presenting cells, natural killer cells, epithelial cells.

We found that air–liquid interface cultures of primary human bronchial epithelial cells (BECs) and an immortalized BEC model (Cdk4/hTERT HBEC) produce H 2O 2 in response to histamine. The main source of airway epithelial H 2O 2 is an NADPH dual oxidase, by: Cell Cultures-BET-lA, a human bronchial epithelial cell line transformed by the SV40 virus, and BEAS-2B, a human bronchial epithelial cell line transformed by an adenovirus SV40 hybrid virus (both gifts of J.

Lechner, National Cancer Institute) (15) were cultured in serum-free LHC-9 medium with 25 pg/ml Fungizone. Southern blot analysis shows that stable integration of the transfected genes occurred. The cell line resembles morphologically the basal cells of the normal human bronchial epithelium, and is not tumorigenic in athymic nude mice.

The cells are able to form tubules when grown in a basement membrane like matrix. The BEAS-2B cell line, originally developed by immortalization of normal human bronchial epithelial cells using ADSV40 virus, has been less frequently used at ALI; however there is some literature using BEAS-2B in this system [17, 26].

Although these cells have been separately characterised by techniques such as immunofluorescence and TEER Cited by: Stimulation of human bronchial epithelial cells by IgE-dependent histamine-releasing factor Kazuo Yoneda,1 Kazuhito Rokutan,2 Yoichi Nakamura,1 Hiroaki Yanagawa,1 Shigetada Kondo-Teshima,2 and Saburo Sone1 1Department of Internal Medicine and Molecular Therapeutics and 2Department of Nutritional Physiology, University of Tokushima School of Medicine.

METHODS: Human lung mast cells were cultured with the bronchial epithelial cell line BEAS-2B or plastic control for either 30 min or 16 h and then activated with anti-IgE. Histamine was measured by radioenzymatic assay.

RESULTS: After co-culture for 30 min, IgE-dependent histamine release from HLMC was identical on both BEAS-2B and by: Epithelial cells (EC) are the first cell types to come into contact with external stimuli, such as chemicals and particulate materials.

James S. Lowe BMedSci, BMBS, DM, FRCPath, Peter G. Anderson DVM, PhD, in Stevens & Lowe's Human Histology (Fourth Edition), Epithelial cells are a specialized component of many organs. When attempting to mimic the bronchial epithelium, the two most commonly used and described cell lines are the Calu-3 cells (human airway epithelial cells) and the 16HBE14o- cells (human bronchial epithelial cells) when considering pulmonary drug models usually consist of a cell monolayer cultivated on permeable growth supports, such as Transwells filters, to.

By expressing two genes (hTERT and Cdk4), we have developed a method to reproducibly generate continuously replicating human bronchial epithelial cell (HBEC) lines that provide a novel resource to study the molecular pathogenesis of lung cancer and the differentiation of bronchial epithelial cells.

Twelve human bronchial epithelial biopsy. Thaw the components of the growth kit just prior to adding them to the basal medium. Warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates prior to adding to the basal medium.

Obtain one bottle of Airway Epithelial Cell Basal Medium ( mL) from cold storage.The main histamine physiologic effects on H1 and H2 receptors will be discussed here as an introduction to antihistamines drug therapy and its clinical uses. Histamine physiology: actions on tissues Histamine is an autacoid (molecule secreted locally to increase or decrease the activity of nearby cells) synthesized from the amino acid histidine.The human bronchial epithelial cell line BEAS-2B secretes inflammatory cytokines in response to LPS, TNF-α, or IL-1β.

When BEAS-2B cells were treated with different concentrations of hrHRF, release of IL-8 (Fig. 1, A and C) and GM-CSF (Fig.

1, B and D) were significantly stimulated in a dose- and time-dependent fashion, compared with the Cited by: